纤维化miRNAPCR芯片 Fibrosis miScript miRNA PCR Array

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Fibrosis miScript miRNA PCR Array

纤维化miRNAPCR芯片

 
The Human Fibrosis miScript miRNA PCR Array profiles the expression of 84 miRNAs known to play a role in fibrosis. During tissue remodeling that occurs as part of wound healing, apoptosis removes myofibroblasts, and the deposited extracellular matrix (ECM) is remodeled to resemble the original tissue. Fibrosis occurs when inappropriate tissue remodeling results in excess ECM deposition due to inappropriate survival of myofibroblasts or lack of ECM proteolytic degradation. The inflammation and enhanced TGFβ signaling often present in fibrotic tissues causes cells to differentiate into myofibroblasts via epithelial-to-mesenchymal transition (EMT). miRNAs may play a role in conducting the pathogenesis of fibrosis by modulating the expression of genes involved in ECM remodeling and cell adhesion, inflammation, angiogenesis, TGFβ/CTGF signaling, and EMT. This array includes miRNAs that are differentially expressed during fibrosis and are known or predicted to modulate the expression of pro- and anti-fibrotic genes common to different fibrotic disorders such as systemic sclerosis, as well as liver, cardiac, kidney, and idiopathic pulmonary fibrosis. The profiling results from this array may yield insights into the molecular mechanisms behind the pathogenesis of fibrosis. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green real-time PCR, the expression of a focused panel of miRNAs involved in fibrosis can be easily and reliably analyzed with this miScript miRNA PCR Array.
miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available
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纤维化miRNAPCR芯片用于研究在纤维化中发挥重要作用的84miRNA的表达。在组织重建期，肌成纤维细胞通过细胞凋亡被清除，沉积的细胞外基质（ECM）被重塑，更像原始组织。若组织重塑异常，例如肌成纤维细胞异常生存或者ECM蛋白水解酶降解缺失，都有可能导致过量ECM沉积，则会造成纤维化。在纤维化的组织中，炎症反应和增强的TGF.信号通路，会导致细胞通过上皮间质转化（EMT）分化为肌成纤维细胞。与之相对的另一面，由于ECM降解被加强了，导致慢性损伤的组织重塑异常。芯片包括编码ECM重建酶基因，TGF.信号通路分子和炎症细胞因子，还包括其他与纤维化密切相关的基因。miRNA可能在进行纤维化的发病机制中发挥作用,在ECM重塑和细胞粘附,炎症,血管生成,TGFβ/ CTGF信号,EMT,调节相关基因的表达。。这个芯片包含在纤维化过程差异表达的miRNA，以及已知或预测调节促进和抑制纤维化基因的miRNA,这些基因常见于不同的纤维化疾病如系统性硬化症,以及肝脏、心脏、肾脏、特发性肺纤维化。这个芯片的分析结果将助于了解纤维化发病的分子机制。每张芯片含一个对照组使得分析数据时可以用相对定量ΔΔCT方法评估逆转录效率,基因组DNA污染,和PCR效率。利用这张芯片，通过实时定量PCR，可以简易且可靠地分析纤维化相关miRNA的表达。
 
miScript miRNA PCR芯片仅用于分子生物学应用。本产品不用于疾病的诊断、预防和治疗。
提供96孔板——384 -(4 x 96)板,和100孔板
 
Pro-Fibrotic: miR-142-3p , miR-145-5p, miR-155-5p, miR-199b-5p, miR-21-5p, miR-215, miR-216a-5p, miR-27a-3p, miR-27b-3p, miR-32-5p, miR-338-5p, miR-34a-5p, miR-377-3p, miR-382-5p, miR-192-5p, miR-30a-5p, miR-208a, miR-5692a, miR-5011-5p.
Anti-Fibrotic: let-7d-5p, miR-107, miR-132-3p, miR-133a, miR-141-3p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-18a-5p, miR-194-5p, miR-19a-3p, miR-19b-3p, miR-200a-3p, miR-200b-3p, miR-204-5p, miR-20a-5p, miR-211-5p, miR-26a-5p, miR-26b-5p, miR-29b-3p, miR-335-5p, miR-449a, miR-449b-5p, miR-590-5p, miR-92a-3p.
Extracellular Matrix Remodeling & Cell Adhesion: miR-199a-5p, miR-199b-5p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-449a, miR-21-5p, miR-204-5p, miR-25-3p, miR-7-5p, miR-196a-5p, miR-203a, miR-27b-3p, miR-27a-3p, miR-451a, miR-145-5p, miR-143-3p, miR-1, miR-10a-5p, miR-10b-5p, miR-661, miR-30a-5p, miR-16-5p, miR-217.
Inflammation: let-7d-5p, miR-204-5p, miR-122-5p, miR-146a-5p, miR-199a-5p, miR-155-5p, miR-21-5p, miR-129-5p, miR-142-3p, miR-503-5p.
Angiogenesis: let-7d-5p, miR-378a-3p, miR-372, miR-34a-5p, miR-29b-3p, miR-20a-5p, miR-200b-3p, miR-195-5p, miR-17-5p, miR-16-5p, miR-15b-5p, miR-150-5p, miR-145-5p, miR-126-3p, miR-107, miR-31-5p, miR-375.
Signal Transduction & Transcriptional Regulation: miR-26a-5p, miR-18a-5p, miR-122-5p, miR-133a, miR-20a-5p, miR-203a, miR-19a-3p, miR-17-5p, miR-146a-5p, miR-155-5p, miR-92a-3p, miR-29b-3p, miR-141-3p, miR-29a-3p, miR-204-5p, miR-590-5p, miR-372, miR-302b-3p, miR-19b-3p, miR-21-5p, miR-1, miR-217, miR-23a-3p, miR-328, miR-744-5p, miR-148a-3p, miR-451a, miR-125b-5p, miR-449a, miR-34a-5p, miR-29c-3p, miR-195-5p, miR-192-5p, miR-16-5p, miR-15b-5p, miR-663a, miR-30a-5p, miR-378a-3p, miR-145-5p, miR-146b-5p, miR-27a-3p, miR-324-5p, miR-324-3p, miR-223-3p, miR-491, miR-101-3p, miR-491-5p.
Epithelial-to-Mesenchymal Transition: let-7d-5p, miR-107, miR-155-5p, miR-199a-5p, miR-199b-5p, miR-200a-3p, miR-200b-3p, miR-215, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-382-5p, miR-325, miR-874

工作原理：

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template