表观遗传染色体修饰酶PCR芯片可用于研究与基因组DNA和调节染色体可塑性组蛋白修饰有关的84个关键基因的表达,这些基因包括已知的或推测的。维持甲基化的DNA甲基转移酶,负责CpG双核苷脱甲基酶,催化组蛋白乙酰化、甲基化、磷酸化、泛素化的酶以及去乙酰酶和去甲基化酶都在此芯片上。该芯片还分析编码SET编码域的蛋白,他们都含有一个共同的编码域,用以阐述对一些家族成员的组蛋白甲基转移酶活性。在干细胞的发育晚期成为分化细胞,基因表达改变的发生可以用来调控染色体动力学。肿瘤细胞也展现出相对于正常细胞不同的基因表达谱,这表明在肿瘤生成中染色质修饰和重构的作用。通过实时定量PCR的方法,研究者即能够利用该芯片简单可靠地同时检测与表观遗传染色体修饰酶有关的基因表达。 DNA Methyltransferases: DNMT1, DNMT3A, DNMT3B. Histone Acetyltransferase: ATF2, CDYL, CIITA, CSRP2BP, ESCO1, ESCO2, HAT1, KAT2A (GCN5L2), KAT2B (PCAF), KAT5 (HTATIP), KAT8, KAT7, KAT6A, KAT6B, NCOA1, NCOA3, NCOA6. Histone Methyltransferases: CARM1 (PRMT4), DOT1L, EHMT2, MLL, MLL3, PRMT1, PRMT2, PRMT3, PRMT5, PRMT6, PRMT7, PRMT8, SETDB2, SMYD3, SUV39H1. SET Domain Proteins (Histone Methyltransfesase Activity): ASH1L, MLL3, MLL5, NSD1, SETD1A, SETD1B, SETD2, SETD3, SETD4, SETD5, SETD6, SETD7, SETD8, SETDB1, SUV39H1, SUV420H1, WHSC1. Histone Phosphorylation: AURKA, AURKB, AURKC, NEK6, PAK1, RPS6KA3, RPS6KA5. Histone Ubiquitination: DZIP3, MYSM1, RNF2, RNF20, UBE2A, UBE2B, USP16, USP21, USP22. DNA / Histone Demethylases: KDM1A, KDM5B, KDM5C, KDM4A, KDM4C, KDM6B, MBD2. Histone Deacetylases: HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11.DNA Methyltransferases: DNMT1, DNMT3A, DNMT3B. Histone Acetyltransferase: ATF2, CDYL, CIITA, CSRP2BP, ESCO1, ESCO2, HAT1, KAT2A (GCN5L2), KAT2B (PCAF), KAT5 (HTATIP), KAT8, KAT7, KAT6A, KAT6B, NCOA1, NCOA3, NCOA6. Histone Methyltransferases: CARM1 (PRMT4), DOT1L, EHMT2, MLL, MLL3, PRMT1, PRMT2, PRMT3, PRMT5, PRMT6, PRMT7, PRMT8, SETDB2, SMYD3, SUV39H1. SET Domain Proteins (Histone Methyltransfesase Activity): ASH1L, MLL3, MLL5, NSD1, SETD1A, SETD1B, SETD2, SETD3, SETD4, SETD5, SETD6, SETD7, SETD8, SETDB1, SUV39H1, SUV420H1, WHSC1. Histone Phosphorylation: AURKA, AURKB, AURKC, NEK6, PAK1, RPS6KA3, RPS6KA5. Histone Ubiquitination: DZIP3, MYSM1, RNF2, RNF20, UBE2A, UBE2B, USP16, USP21, USP22. DNA / Histone Demethylases: KDM1A, KDM5B, KDM5C, KDM4A, KDM4C, KDM6B, MBD2. Histone Deacetylases: HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11. |