树突和抗原呈递细胞PCR芯片可用于研究与树突细胞活化和成熟相关的84个基因的表达。与树突细胞活化和成熟相关的基因如细胞因子、趋化因子及其受体以及其他相关的细胞表面受体和信号转导分子包含此芯片上。参与抗原摄取、处理和显示的基因也在此芯片上。除了功能定义,芯片上的许多基因在成熟的树突细胞高表达或在细胞分化有显著的变化。通过实时定量PCR的方法,研究者即能够利用该芯片简单可靠地同时检测与树突和抗原呈递细胞相关的基因表达。 Antigen Uptake: CD44, CDC42, ICAM1, ICAM2, RAC1, TAP2 (ABCB3). Antigen Presentation: CCL19, CD1A, CD1B, CD1C, CD1D, CD209, CD28, CD4, CD40 (TNFRSF5), CD40LG (TNFSF5), CD74, CD80, CD86, CD8A, HLA-A, HLA-DMA, HLA-DPA1, TAPBP, THBS1. Dendritic Cell Chemotaxis: CCL19, CCL5 (RANTES), CCR1, CCR2, CCR5, CXCR1 (IL8RA), CXCR4. Dendritic Cell Differentiation: CSF2 (GM-CSF), LYN, RELB, TGFB1. Cytokines: CCL11 (Eotaxin), CCL13 (MCP-4), CCL16 (HCC4), CCL19, CCL2 (MCP-1), CCL3 (MIP-1A), CCL5 (RANTES), CCL7 (MCP-3), CCL8 (MCP-2), CSF2 (GM-CSF), CXCL1, CXCL10 (INP10), CXCL12 (SDF1), CXCL2, FLT3LG, IFNG, IL10, IL12A, IL12B, IL16, IL2, IL6, IL8, MIF, TNF, TNFSF11. Cytokine Receptors: CCR1, CCR2, CCR3, CCR5, CSF1R, CXCR1 (IL8RA), CXCR4, FLT3, ERBB2 (HER2). Other Cell Surface Receptors: CD2, CD40 (TNFRSF5), FCER1A, FCER2, FCGR1A, LRP1, TLR1, TLR2, TLR7, TLR9, VCAM1. Signal Transduction: CDKN1A (p21CIP1/WAF1), CEBPA, CLEC4C, FAS (TNFRSF6), IRF7, IRF8, ITGAM, ITGB2, LYN, NFKB1, PTPRC, RELA, RELB, STAT3, TGFB1. |