类mTOR信号通路PCR芯片可用于研究mTOR信号通路中的84个关键基因的表达。mTOR是一类丝氨酸/酪氨酸蛋白激酶，整合了来自各方面的信号（如营养，荷尔蒙，生长因子以及细胞压力等）来调节细胞的生长，代谢以及生存。mTOR激酶可以形成两类蛋白复合物mTORC1 和mTORC1。 mTORC1调控多个细胞生物合成过程（蛋白合成细胞周期进程，细胞生长以及增殖）。mTORC2能激活AKT，对于细胞的增殖，迁移和存活很重要。该芯片中包含mTORC1和mTORC2复合物中的成员蛋白基因，调控mTOR反应的因子，及受mTOR调控下游基因。利用实时定量PCR，研究者可以方便并且可信地对与mTOR相关的基因进行同时检测。
mTOR Complexes: mTORC1: MLST8, MTOR, RPTOR. mTORC2: MLST8, MAPKAP1 (SIN1), MTOR, RICTOR. mTOR Upstream Regulators: mTORC1 Positive Regulators: AKT1, AKT2, AKT3, HRAS, IGF1, IKBKB, INS, INSR, IRS1, MAPK1, MAPK3, PDPK1, PIK3C3, PIK3CA, PIK3CB, PIK3CD, PIK3CG, PLD1, PLD2, RHEB, RPS6KA1, RPS6KA2, RPS6KA5, RRAGA, RRAGB, RRAGC, RRAGD, TELO2. mTORC2 Positive Regulators: AKT1, AKT2, AKT3, MAPK1, MAPK3, PDPK1, PIK3C3 (VPS34), PIK3CA, PIK3CB, PIK3CD, PIK3CG, RHEB,RPS6KA1, RPS6KA2, RPS6KA5. mTORC1 Negative Regulators: AKT1S1 (PRAS40), CAB39, CAB39L, DDIT4, DDIT4L, DEPTOR, FKBP1A (FKBP12), FKBP8, IGFBP3, PRKAA1 (AMPK), PRKAA2, PRKAB1, PRKAB2, PRKAG1, PRKAG2, PRKAG3, PTEN, STK11 (LKB1), STRADB, TP53, TSC1, TSC2, YWHAQ (14-3-3θ). mTORC2 Negative Regulators: CAB39, CAB39L, DDIT4 (REDD1), DDIT4L (REDD2), DEPTOR, PRKAA1 (AMPK), PRKAA2, PRKAB1, PRKAB2, PRKAG1, PRKAG2, PRKAG3, STK11 (LKB1), STRADB, TSC1, TSC2. mTOR Downstream Effectors: mTORC1 Positive Regulation: CHUK (IKKa), EIF4B, EIF4E, HIF1A, IKBKB, RPS6, RPS6KB1, RPS6KB2, TP53, VEGFA, VEGFB, VEGFC. mTORC2 Positive Regulation: AKT1, CDC42, GSK3B, HSPA4, ILK, MYO1C, PRKCA, PRKCB, PRKCE, PRKCG, RHOA, RPS6KB1, SGK1. mTORC1 Negative Regulation: EIF4EBP1, EIF4EBP2, PPP2CA, PPP2R2B, PPP2R4, TP53, ULK1, ULK2. Cellular Processes: Amino Acid Response: PIK3C3 (VPS34), RRAGA, RRAGB, RRAGC, RRAGD. Angiogenesis: CHUK (IKKa), DDIT4 (REDD1), DDIT4L (REDD2), HIF1A, IKBKB, VEGFA, VEGFB, VEGFC. Autophagy: PIK3C3 (VPS34), ULK1, ULK2. Cytoskeletal Organization: CDC42, PRKCA, PRKCB, PRKCE, PRKCG, RHOA. Growth Factor Response: HRAS, IGF1, IGFBP3, MAPK1, MAPK3, RPS6KA1, RPS6KA2, RPS6KA5. Energy Stress: PRKAA1 (AMPK), PRKAA2, PRKAB1, PRKAB2, PRKAG1, PRKAG2, PRKAG3. Insulin Signaling: AKT1, AKT2, AKT3, INS, INSR, IRS1, PDPK1, PIK3CA, PIK3CB, PIK3CD, PIK3CG, TP53. Lipid Metabolism: PLD1, PLD2. Translation: EIF4B, EIF4E, EIF4EBP1, EIF4EBP2, PPP2CA, PPP2R2B, PPP2R4, RPS6, RPS6KA1, RPS6KA2, RPS6KA5, RPS6KB1, RPS6KB2. |
