cAMP/Ca2+ 通路发现者PCR芯片 cAMP/Ca2+ PathwayFinder PCR
cAMP/Ca2+ 通路发现者PCR芯片 cAMP/Ca2+ PathwayFinder PCR
cAMP/Ca2+ 通路发现者PCR芯片cAMP/Ca2+ PathwayFinder PCR Array
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芯片可用于研究参与cAMP/Ca2+应答的84的关键基因的表达。这一芯片所包含的基因，其启动子区域具有SRE或SRE-like增强子序列、CRE增强子序列或其他Ca2+应答元素。通过实时定量PCR的方法，研究者即能够利用该芯片简单可靠地同时检测cAMP/Ca2+信号通路相关基因的表达。 Genes whose promoters contain SRE or SRE-like enhancer sequences: CNN1 (calponin), CYR61, EGR1, EGR2, FOS, FOSB, HSPA4 (hsp70), JUNB, NR4A2 (Nur77), S100A6 (Calcyclin), SCG2 (secretogranin II), SRF, THBS1 (TSP-1), VCL. Genes whose promoters contain the CRE enhancer sequence: Neuropeptides / Neurotransmitters: ADRB1 (b1 adrenergic receptor), CGA (Chorionic gonadotropin A), CHGA (Chromogranin), GCG (Glucagon), GIPR, INHBA (Inhibin A), KCNA5 (KV1.5), NOS2 (Inducible nitric oxide synthase), PENK (Enkephalin), PRL, S100A12 (CGRP), S100G, SCG2, SLC18A1 (Vesicular monoamine transporter), SST (Somatostatin), SSTR2 (Somatostatin receptor 2), TACR1 (Substance P receptor), TH(Tyrosine hydroxylase), VIP. Cell Cycle / Cell Survival / DNA Repair: BCL2, BRCA1, CCNA1 (CyclinA), CCND1 (CyclinD1), CDK5, CDKN2B (p15IND4b), GEM, NF1, PCNA, PMAIP1 (NoxA), PPP1R15A (GADD34), RB1. Growth Factor: AREG (Amphiregulin), BDNF, CTF1 (Cardiotrophin), FGF6, TGFB3, TNF. Signaling: DUSP1 (Ptpn16), HSPA5 (Grp78), PLN (Phospholamban), PPP2CA (Protein phosphatase 2A alpha), PRKAR1A (Proteinkinase A regulatory subunit), SGK1. Transcription: ATF3, CREB1, CREM, EGR1, EGR2, FOS, JUND, MAF, NR4A2 (Nur77), PER1, POU1F1 (Pit-1), POU2AF1 (OCA-B), STAT3. Metabolism: AHR, AMD1, ENO2, HK2, LDHA, PCK2, SOD2. Immune Regulation: IL2, IL6, MIF, PTGS2 (Cox-2). Genes whose promoters contain other Ca2+ responsive elements: CALB1 (calbindin 1), CALB2 (calbindin 2), CALM1 (Calmodulin 1), CALR (Calreticulin), DDIT3 (GADD153), NCAM1, NPY, PLAT (tPA). |
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