| Human Aging PCR Array |
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| The Human Aging RT2 Profiler PCR Array profiles the expression of 84 genes altered during aging, a major biological process and a risk factor for many diseases. To gain new insights into the process of aging and identify potentially important genes and biomarkers, many microarray studies have been conducted in several species, including humans, either by comparing various young and old tissues or by comparing samples across the organism's lifespan. Characterization of the associated biological processes has found that age-related gene expression changes involve alterations in the expression of genes related to apoptosis, cell cycle, cell senescence, and inflammation, as well as mitochondrial genes. The other common denominators of aging include altered expression of genes linked to epigenetic alterations, genomic instability, telomere attrition, and proteostasis - the stabilization of correctly folded proteins. These molecular markers and processes reflect a combination of degeneration and transcriptional responses to aging. Analyzing the expression of these genes can provide a better understanding of how transcriptional changes relate to the aging process. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification and assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes related to aging with this array.
The RT² Profiler PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. |
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Genomic Instability: BUB1B, MRPL43, POLRMT, TFAM, TFB1M, TFB2M, ZMPSTE24.
Telomere Attrition: POT1, RAP1A, TERF1, TERF2, TINF2, TPP1.
Mitochondrial Dysfunction: MRPL43, NDUFB11, POLRMT, SIRT1, SIRT3, SIRT6, TFAM, TFB1M, TFB2M.
Proteostasis: ARL6IP6, BUB1B, FOXO1, HSF1, HSF1, JAKMIP3, RNF144B, SIRT1, TXNIP, VPS13C.
Laminopathies: LMNA, LMNB1, LMNB2, ZMPSTE24.
Neurodegeneration & Synaptic Transmission: CALB1, GFAP, MBP, SCN2B, SNAP23.
Epigenetic Alterations: ARID1A, SIRT1, SIRT3, SIRT6.
DNA Binding: ARID1A, ELP3, EP300, FBXL16, ZBTB10, ZFR, ZNF25.
RNA Binding: ELAVL1, LSM5, ZFR.
Inflammatory response: ANGEL2, ANXA3, ANXA5, C1QA, C1QB, C1QC, C1S, C3, C3AR1, C5AR1, CCR1, CD14, CD163, CFH, CX3CL1, CXCL16, FCER1G, FCGBP, FCGR1A, FCGR2A, FCGR3B, GFAP, LTF, LYZ, MBP, PANX1, S100A8, S100A9, TMEM135, TMEM33, TLR2, TLR4, TOLLIP.
Apoptosis: CASP1, CLU, EP300, PDCD6, TOLLIP.
Cellular Senescence: CDKN1C, VWA5A, WRN.
Cell cycle: BUB1B, CDKN1C.
Cytoskeleton: COL1A1, COL3A1, EML1.
Oxidative Stress: EP300, GSTA1.
Transcriptional regulation: ARID1A, EP300, FOXO1, HSF1, PHF3, SMAD2. |
