Notch信号靶标PCR芯片可用于研究Notch信号转导的84个关键应答基因的表达。Notch信号通路是一个传统的发育途径，参与细胞间通讯，细胞命运，细胞凋亡和发育。Notch信号通路首先在果蝇中发现，哺乳动物同源的Notch信号通路稍晚些被确定。来自Delta及Jagged/Serrate的配体可以激活与膜结合的Notch受体，诱导Notch胞内结构域断裂。这种胞内结构域与RBPJ家族的转录因子以及共激活因子和辅阻遏物的相互作用来启动靶基因的转录。Notch信号通路激活的输出依赖于细胞内容物，在适当的时空调节激活是正常的胚胎发育过程所必须的。Notch信号通路失调可能会导致癌症以及遗传发育障碍。通过如ChIP和基因表达研究等实验技术，很多经典的Notch通路靶标基因都已经被鉴定出来了。该芯片包含了Notch经典信号通路的转录因子和研究验证得到的高关联性靶标基因，通过该芯片得到的结果可用于Notch信号传导激活和抑制研究。通过实时定量PCR的方法，研究者即能够利用该芯片简单可靠地同时检测Notch调节的细胞活动相关基因的表达。
Cell Fate Commitment: DKK1, HES1, HES5, JAG1, NODAL, NOTCH1, NOTCH3, PAX6, RUNX2, SOX9. Developmental Processes: Embryonic Development: DKK1, EDN1, EFNB1, FOXC1, FOXD3, FOXF1, HES1, HES7, MYF5, NODAL, NOTCH1, PBX1, PCDH8, PDGFRA, PDGFRB, RBPJ, TCF15, VEGFA, WNT4, WNT5A. Muscle Development: EDN1, EGR3, FOXC1, HBEGF (DTR), HEY2, IGFBP3, JAG1, MYF5, NOTCH1, PDGFB, PDGFRB, TNC. Epidermis Development: JAG1, KRT14, NOTCH1, PTGS2 (COX2), SNAI1 (SNAIL), SOX9, TCF15. Immune System Development: ID2, JAG1, JUN, KITLG, PBX1, RUNX1 (AML1), SGPL1, VEGFA. Nervous System Development: BDNF, CCND2, DKK1, DTX1, EFNA1, EFNB1, EGR3, FABP7, FOXC1, HES1, HES5, HEY1, HEY2, HEYL, ID3, ID4, JAG1, KALRN, NES, NODAL, NOTCH1, NOTCH3, PAX6, PBX1, RBPJ, RND1, VEGFA. Vasculature Development: EDN1, EFNA1, FLT1, FOXC1, FOXF1, HEY2, ID1, JAG1, JUN, NODAL, NOTCH1, RBPJ, RUNX1 (AML1), SGPL1, VEGFA. Kidney Development: ADAMTS1, BDNF, FOXC1, PBX1, PDGFRB, SGPL1. Other Development: NRARP, S1PR3. Signal Transduction: Notch Pathway: CREBBP (CBP), DTX1, FOXC1, HES1, HES7, HEY1, HEY2, HEYL, JAG1, LFNG, NOTCH1, NOTCH3, RBPJ, RBPJL, SNW1 (SKIIP). WNT Pathway: CCND1, DKK1, FRZB (FRP-3), FZD5, WISP1, WNT4, WNT5A, WNT6. Apoptosis: ABL1, BDNF, FOXC1, GADD45B, ID3, IGFBP3, JUN, KALRN, NOTCH1, PTCRA, PTGS2 (COX2), SGPL1, SOCS3, SOX9, VEGFA. Cytokines: CX3CL1, CXCL12 (SDF1), CXCL2, IL33, NAMPT. Transcription Factors: CBFA2T3CLOCK, CREBBP (CBP), EGR3, FOXC1, FOXD3, FOXF1, HEY1, HEY2, HEYL, JUN, MSC, MYF5, NOTCH1, PAX6, PBX1, RBPJ, RBPJL, RUNX1 (AML1), RUNX2, SOX9, TCF15. Other Genes: FJX1, GPSM2, HSPB8, MARK1, RHOV, TEC. |
